Mouse HMGB-1(High Mobility Group Protein B1) CLIA Kit (E-CL-M0419)

Name: Mouse HMGB-1(High Mobility Group Protein B1) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0419
Price: 180.0 USD
Availability: InStock

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Mouse HMGB-1(High Mobility Group Protein B1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse HMGB-1 in samples.No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51306.000	52912.000	52109.000	52081
250	22487.000	22933.000	22710.000	22682
125	10760.000	9624.000	10192.000	10164
62.5	4364.000	4728.000	4546.000	4518
31.25	2558.000	2214.000	2386.000	2358
15.63	1482.000	1264.000	1373.000	1345
7.81	775.000	813.000	794.000	766
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.080	64.700	219.310	25.600	62.530	206.960
Standard deviation	2.400	5.230	13.330	2.910	4.690	22.770
CV（%）	9.970	8.080	6.080	11.370	7.500	11.000

Recovery

The recovery of Mouse HMGB-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-110	100
EDTA plasma （n=8）	92-107	100
Cell culture media （n=8）	90-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse HMGB-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-114	98-114	88-100
1:2	Average	104	105	93
1:4	Range	103-119	100-111	100-115
1:4	Average	109	105	109
1:8	Range	85-100	97-107	85-99
1:8	Average	92	102	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

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